Dissection of Palate Tissue from Adult Murine Model: A Surgical Procedure to Harvest Hard Palate and Soft Palate from Oral Cavity of Adult Mouse

Published: April 30, 2023

Abstract

Source: Xuan Ngo, Y. et al. Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse PalateJ. Vis. Exp. (2021)

This video demonstrates a surgical procedure to dissect the palate tissue from a murine model.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Dissection of Palate Tissue from Adult Mouse Sacrifice an adult C57BL/6J mouse (either male or female) by cervical dislocation in compliance with the facility's regulations relating to animal welfare. Remove the hair around the mouth with a shaver. Using scissors, cut from the cheek towa…

Representative Results

Figure 1: Overview of the dissection procedure and isolation of mouse oral keratinocytes. (A) Schematic representation of the mouse oral cavity. (B) Instruments used to dissect the palate and isolate mouse oral keratinocytes. (C) Brightfield image of the mouse palate. Scale bar: 100 µm. (D) Summary of th…

Offenlegungen

The authors have nothing to disclose.

Materials

Iris Scissors Muromachi Kikai 14090-09
Medetomidine Nippon Zenyaku Kogyo Domitor
Midazolam Astellas Pharma Dormicum
No.15 Disposable scalpel Feather 219AABZX00136000
Butorphanol Meiji Seika Pharma Vetorphale
Half-curved forceps BRC Bio Research Center PRI13-3376
Povidone-iodine Y's Square 872612
Raspatorium #14 AS ONE 8-4599-01
Fine forceps BRC Bio Research Center PRI13-3374
Antibiotic-Antimycotic 100x Gibco 15240062
15 mL sterile conical tube Falcon 352096
Cotton AS ONE 63-1452-97
EpiLife Defined Growth Supplement (EDGS)  Gibco  S0120

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Diesen Artikel zitieren
Dissection of Palate Tissue from Adult Murine Model: A Surgical Procedure to Harvest Hard Palate and Soft Palate from Oral Cavity of Adult Mouse. J. Vis. Exp. (Pending Publication), e20740, doi: (2023).

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