Co-culturing BC Cells: Culturing Breast Cancer Cells with Bone Fragments to Study Cancer Cell Proliferation
Published: April 30, 2023
Abstract
Source: Templeton, Z. S., et al. Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche. J. Vis. Exp (2015).
This video describes the culturing of breast cancer cells adjacent to femur bone explants to measure cancer cell proliferation and colonization in a human bone tissue explant model system.
Protocol
1. Co-culturing Breast Cancer Cells Adjacent to Bone Fragments to Measure Breast Cell Proliferation Using BLI Prior to the experiment, prepare a suspension containing 2 x 105 breast cancer cells/50 µl, and prepare plugs of bone wax for immobilizing bone fragments in culture. Cut a P200 micropipette tip into 3 pieces and use the narrow end of the largest piece in a “cookie cutter” manner to cut small (~35 mg) pieces of bone wax. Use the wide end of the smallest piece to eject t…
Offenlegungen
The authors have nothing to disclose.
Materials
| DMEM (1X) + GlutaMAX-l | Life Technologies | 10569-010 | Supplement all DMEM with 10% fetal bovine serum and 1% Pen/ Strep |
| Fetal bovine serum | Life Technologies | 16000-044 | |
| Penicillen Streptomycin | Life Technologies | 15140-122 | |
| D-luciferin firefly, potassium salt 5 g (30 mg/ml) | Life Technologies | L-8220 | |
| 6 Well TC-Treated Cell Culture Cluster | Corning Incorporated | 3516 | |
| Friedman-Pearson Rongeur | Fine Science Tools | 16021-14 | |
| Bone wax | Surgical Specialties Corporation | 903 | |
| IVIS 50 Imaging Platform | Caliper Life Sciences/PerkinElmer | ||
| MCF-7 breast cancer cells | ATCC | HTB-22 | |
| MDA-MB-231 breast cancer cells | ATCC | HTB-26 |
Diesen Artikel zitieren
Co-culturing BC Cells: Culturing Breast Cancer Cells with Bone Fragments to Study Cancer Cell Proliferation. J. Vis. Exp. (Pending Publication), e20215, doi: (2023).