Open Field Behavior Test: A Method to Assess General Locomotion and Exploration Habits
Published: April 30, 2023
Abstract
Source: Osmon, K. J., et al. Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice. J. Vis. Exp. (2018).
This video describes the open field behavior test used to examine general locomotion and exploration habits in rodents.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. NOTE: Murine behavioral studies are largely sex/age/strain-dependent, and therefore it is essential to collect the proper time points and to remain consistent across experimental groups. Environmental factors (e.g., scents, noise, temperature, humidity lighting, etc.) greatly affect behavior and anxiety in mice. Try…
Materials
| Square Open Field 45 x 45 x 40 cm (grey color). Other floor color available: white, black. | Harvard Aparatus (PanLab) | 760190 | |
| Open Field Preconfigured Module | Harvard Aparatus (PanLab) | 760688 | |
| Smart V3.0 Software Platform | Harvard Aparatus (PanLab) | 760681 | |
| Video converter (analog/digital) | Harvard Aparatus (PanLab) | 760262 | |
| Roof fixing sys for camera | Harvard Aparatus (PanLab) | 760277 | |
| Analog Sony Camera & lens NTSC | Harvard Aparatus (PanLab) | 760505 |
Tags
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Open Field Behavior Test: A Method to Assess General Locomotion and Exploration Habits. J. Vis. Exp. (Pending Publication), e20062, doi: (2023).