Transfer and Rearing of Butterfly Eggs on Agar-Based Diet Until Adult Emergence (Video) | JoVE
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Artificial Diet Preparation Recipe:
Ingredients:
– 15 G Fine Mesh Agar
– 400 Ml Distilled Water
– 400 Ml Faucet-temperature Distilled Water
– 5 Ml Flaxseed Oil
Instructions:
1. Weigh All The Dry IngredientsExcept AgarInto One Container.
2. Place The Dry Ingredient Mixture Into A Blender And Add 5 Ml Of Flaxseed Oil.
3. In A 1-liter BeakerMix 15 G Of Fine Mesh Agar With 400 Ml Of Distilled Water.
4. Heat The Agar-water Mixture In A Microwave Until Close To BoilingWith Fine Bubbles Throughout.
5. Add 400 Ml Of Faucet-temperature Distilled Water To The Agar Mixture To Lower The Temperature.
6. Add The Agar Mixture To The Blender And Thoroughly Mix.
7. Pour The Diet Mixture Into 4-ounce Diet CupsEnsuring The Diet Covers The Bottom Of Each Cup.
8. Allow The Diet To CoolThen Place Lids On The Cups And Label Them With The Diet Type.
9. Stack The Labeled Cups On Trays And House Them In A 24°C Climate Chamber
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Transfer and Rearing of Butterfly Eggs on Agar-Based Diet Until Adult Emergence. J. Vis. Exp. (Pending Publication), e200339, doi:10.3791/200339 (2023).