Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein (Video) | JoVE

Published: June 30, 2023

doi:

10.3791/200248

Bree R. Streather, Karen Baker, Tara A. Eastwood, Daniel P. Mulvihill

¹School of Biosciences,University of Kent

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Keywords: Recombinant Vesicle Bacterial Culture Soluble Protein VNP Sequence Tag Lysogeny Broth Terrific Broth IPTG Centrifugation Filtration Vesicle Concentration Sonication BL21DE3 Escherichia Coli MNeongreen SDS-PAGE

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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein. J. Vis. Exp. (Pending Publication), e200248, doi:10.3791/200248 (2023).

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein (Video) | JoVE

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