JOVE JOURNAL

Biotechnik

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Journal (Bioengineering)

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Kaizhe Chen*¹, Haoyi Chen*¹, Lianfu Deng¹, Kai Yang¹, Jin Qi¹

¹Department of Orthopedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital,Shanghai Jiao Tong University School of Medicine

Journal (Bioengineering)

Semi-Automated Mechanical Dissociation of Mouse Tissues to Generate Single-Cell Suspensions

Mayowa Amosu¹, Andrew J. Gregory², John D. Murtagh², Nitay Pavin², Carson Taylor Meyers², Juan Grano de Oro Fernandez¹, Kaitlyn Moore¹, Katharina Maisel¹

¹Fischell Department of Bioengineering,University of Maryland, ²UMD Terrapin Works,University of Maryland

Journal (Bioengineering)

Mechanical Dissociation of Tissues for Single Cell Analysis Using a Motorized Device

Mayowa Amosu¹, Andrew J. Gregory², John D. Murtagh², Nitay Pavin², Carson Taylor Meyers², Juan Grano de Oro Fernandez¹, Kaitlyn Moore¹, Katharina Maisel¹

¹Fischell Department of Bioengineering,University of Maryland, ²UMD Terrapin Works,University of Maryland

Journal (Bioengineering)

Superior Auto-Identification of Trypanosome Parasites by Using a Hybrid Deep-Learning Model

Veerayuth Kittichai¹, Morakot Kaewthamasorn², Suchansa Thanee², Thanyathep Sasisaowapak³, Kaung Myat Naing³, Rangsan Jomtarak⁴, Teerawat Tongloy³, Santhad Chuwongin³, Siridech Boonsang⁵

¹Faculty of Medicine,King Mongkut’s Institute of Technology Ladkrabang, ²Veterinary Parasitology Research Unit, Faculty of Veterinary Science,Chulalongkorn University, ³College of Advanced Manufacturing Innovation,King Mongkut’s Institute of Technology Ladkrabang, ⁴Faculty of Science and Technology,Suan Dusit University, ⁵Department of Electrical Engineering, School of Engineering,King Mongkut’s Institute of Technology Ladkrabang

Journal (Bioengineering)

Training Process CiRA CORE Platform and Object Detection Model Evaluation to Identify and Classify the Protozoan Trypanosome Species

Veerayuth Kittichai¹, Morakot Kaewthamasorn², Suchansa Thanee², Thanyathep Sasisaowapak³, Kaung Myat Naing³, Rangsan Jomtarak⁴, Teerawat Tongloy³, Santhad Chuwongin³, Siridech Boonsang⁵

¹Faculty of Medicine,King Mongkut’s Institute of Technology Ladkrabang, ²Veterinary Parasitology Research Unit, Faculty of Veterinary Science,Chulalongkorn University, ³College of Advanced Manufacturing Innovation,King Mongkut’s Institute of Technology Ladkrabang, ⁴Faculty of Science and Technology,Suan Dusit University, ⁵Department of Electrical Engineering, School of Engineering,King Mongkut’s Institute of Technology Ladkrabang

Journal (Bioengineering)

Image Classification as Model Training and Model Evaluation to Identify and Classify the Protozoan Trypanosome Species

Veerayuth Kittichai¹, Morakot Kaewthamasorn², Suchansa Thanee², Thanyathep Sasisaowapak³, Kaung Myat Naing³, Rangsan Jomtarak⁴, Teerawat Tongloy³, Santhad Chuwongin³, Siridech Boonsang⁵

¹Faculty of Medicine,King Mongkut’s Institute of Technology Ladkrabang, ²Veterinary Parasitology Research Unit, Faculty of Veterinary Science,Chulalongkorn University, ³College of Advanced Manufacturing Innovation,King Mongkut’s Institute of Technology Ladkrabang, ⁴Faculty of Science and Technology,Suan Dusit University, ⁵Department of Electrical Engineering, School of Engineering,King Mongkut’s Institute of Technology Ladkrabang

Journal (Bioengineering)

Hybrid (Detection and Classification) as Model Testing to Identify and Classify the Protozoan Trypanosome Species

Veerayuth Kittichai¹, Morakot Kaewthamasorn², Suchansa Thanee², Thanyathep Sasisaowapak³, Kaung Myat Naing³, Rangsan Jomtarak⁴, Teerawat Tongloy³, Santhad Chuwongin³, Siridech Boonsang⁵

¹Faculty of Medicine,King Mongkut’s Institute of Technology Ladkrabang, ²Veterinary Parasitology Research Unit, Faculty of Veterinary Science,Chulalongkorn University, ³College of Advanced Manufacturing Innovation,King Mongkut’s Institute of Technology Ladkrabang, ⁴Faculty of Science and Technology,Suan Dusit University, ⁵Department of Electrical Engineering, School of Engineering,King Mongkut’s Institute of Technology Ladkrabang

Journal (Bioengineering)

Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (cPCR)

M. Reza Roushan¹, Chuchi Chen¹, Parisa Ahmadi¹, Valerie C. A. Ward¹

¹Department of Chemical Engineering,University of Waterloo

Journal (Bioengineering)

Agrobacterium tumefaciens-Mediated Genetic Engineering of Green Microalgae, Chlorella vulgaris

M. Reza Roushan¹, Chuchi Chen¹, Parisa Ahmadi¹, Valerie C. A. Ward¹

¹Department of Chemical Engineering,University of Waterloo

Journal (Bioengineering)

Preparation of Electrocompetent Agrobacterium tumefaciens Culture for Microalgal Genetic Engineering

M. Reza Roushan¹, Chuchi Chen¹, Parisa Ahmadi¹, Valerie C. A. Ward¹

¹Department of Chemical Engineering,University of Waterloo

Journal (Bioengineering)

Electroporation of Agrobacterium tumefaciens for Genetic Engineering of Microalgal Cells

M. Reza Roushan¹, Chuchi Chen¹, Parisa Ahmadi¹, Valerie C. A. Ward¹

¹Department of Chemical Engineering,University of Waterloo

Journal (Bioengineering)

Calculation of the Differential Transformation for Cross-Device Co-Registration of Two Preclinical Imaging Modalities

Mirko Thamm^1,2, Justin J. Jeffery³, Yapei Zhang⁴, Bryan R. Smith⁴, Stephen Marchant⁵, Fabian Kiessling^1,6, Felix Gremse^1,2

¹Institute for Experimental Molecular Imaging,RWTH Aachen University, ²Gremse-IT GmbH, ³Carbone Cancer Center,University of Wisconsin, ⁴Department of Biomedical Engineering and Institute for Quantitative Health Science and Engineering,Michigan State University, ⁵MediLumine Inc., ⁶Fraunhofer Institute for Digital Medicine MEVIS

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