JOVE JOURNAL

Biochemie

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Journal (Biochemistry)

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Gabriel R. Martins*^1,2, Felipe L. Brum*^1,2,3, Davi Marconi Miranda Carvalho da Silva*^1,2, Livia C. Barbosa³, Ronaldo Mohana-Borges³, Ayla Sant'Ana da Silva^1,2

¹Laboratório de Biocatálise (LABIC),Instituto Nacional de Tecnologia, ²Departamento de Bioquímica, Instituto de Química,Universidade Federal do Rio de Janeiro, ³Centro de Espectrometria de Massas de Biomoléculas, Instituto de Biofísica Carlos Chagas Filho,Universidade Federal do Rio de Janeiro

Journal (Biochemistry)

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Késsia Caroline Alves^1,2, Yury Oliveira Chaves^1,3,4, Maria Edilene Almeida^1,4, Maria Gabriella Vasconcelos^1,4, Paulo Afonso Nogueira^1,3,6, Joyce Melo^1,9, Jéssica Marques⁸, Juliana Pavan Zuliani⁷, Charles Nunes Boeno⁷, Mauro Valentino Paloschi⁷, Rachele Isticato⁵, Ezio Ricca⁵, Luís André Mariúba^1,2,3,6

¹Laboratório de diagnóstico e controle de doenças infecciosas na Amazônia,Instituto Leônidas e Maria Deane - Fiocruz Amazônia, ²Programa de Pós-Graduação em Biotecnologia, Instituto de Ciências Biológicas,Universidade Federal do Amazonas (UFAM), ³Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro - Fiocruz Amazônia, ⁴Programa de Pós-graduação em Ciências Aplicadas à Hematologia PPGH-UEA/HEMOAM, ⁵Department of Biology,Federico II University, ⁶Programa de Pós-Graduação em Imunologia Básica e Aplicada, Instituto de Ciências Biológicas,Universidade Federal do Amazonas (UFAM), ⁷Laboratório de Imunologia Celular Aplicada à saúde, Fundação Oswaldo Cruz,FIOCRUZ Rondônia, ⁸Centro Multiusuário para Análises de Fenômenos Biomédicos (CMABio-UEA),Universidade Estadual do Amazonas, ⁹Universidade Federal do Amazonas (UFAM)

Journal (Biochemistry)

Generating Submitochondrial Vesicles from Saccharomyces cerevisiae Mitochondria to Isolate Mitochondrial Contact Sites

Siavash Khosravi*^1,2, Johanna Frickel*¹, Max E. Harner¹

¹Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, ²Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Journal (Biochemistry)

Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

Siavash Khosravi*^1,2, Johanna Frickel*¹, Max E. Harner¹

¹Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, ²Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Journal (Biochemistry)

An Improved Method to Isolate Mitochondrial Contact Sites

Siavash Khosravi*^1,2, Johanna Frickel*¹, Max E. Harner¹

¹Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, ²Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Journal (Biochemistry)

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Pavan S. Krishnan^1,2, Fernando T. Zamuner^1,3, Carolyn M. Jenks¹, Johnny Y. Xie⁴, Lisa Zhang⁵, Mohammed Lehar¹, Neal S. Fedarko⁶, Mariana Brait^1,3, John P. Carey¹

¹Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, ²Virginia Commonwealth University School of Medicine, ³Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, ⁴Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, ⁵Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, ⁶ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine

Journal (Biochemistry)

An Open-Source Framework for Mass Calculation of Antibody-Based Therapeutic Molecules

Thomas Harkins^1,2, Li Cao¹, Kshitij Khatri^1,3

¹GlaxoSmithKline, ²Merck, ³Moderna

Journal (Biochemistry)

Running the mAbscale Application to Calculate the Masses of Monoclonal Antibody-Based Protein Therapeutics

Thomas Harkins^1,2, Li Cao¹, Kshitij Khatri^1,3

¹GlaxoSmithKline, ²Merck, ³Moderna

Journal (Biochemistry)

Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets

Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

Journal (Biochemistry)

Image Acquisition of Lipid Droplets and Analysis

Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

Journal (Biochemistry)

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment

Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

Journal (Biochemistry)

Fibrinolytic Activity of the Fibrinolytic Enzyme Purified from Sipunculus nudus

Mingqing Tang^1,2, Hongjun Lin¹, Chengjia Hu¹, Hui Yan^1,2

¹Medicine School,Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

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