JOVE JOURNAL

Entwicklungsbiologie

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Journal (Developmental Biology)

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

Qihong Zheng*¹, Xiaoxiang Xiang*¹, Yan Yan³, Huapeng H. Yu

¹School of Life Sciences,SUSTech University, ²Shenzhen PKU-HKUST Medical Center, ³Division of Life Science,Hong Kong University of Science and Technology

Journal (Developmental Biology)

Injecting Fluorescently Labeled Antibodies in Live Drosophila Embryos and Imaging of Low Abundance Proteins

Qihong Zheng*¹, Xiaoxiang Xiang*¹, Yan Yan³, Huapeng H. Yu

¹School of Life Sciences,SUSTech University, ²Shenzhen PKU-HKUST Medical Center, ³Division of Life Science,Hong Kong University of Science and Technology

Journal (Developmental Biology)

Culturing H9-Embryonic Stem Cells for Generation of Human Neural Retina

Wen Li*^1,2, Hongyu Li*³, Hao Yan, Lixiong Gao, Xiaoling Wang, Lingping Zhao, Yuhan Yan², Zi Ye, Jiafei Xi, Wen Yue, Zhaohui Li²

¹Medical School of Chinese PLA, ²Senior Department of Ophthalmology,The Third Medical Center of Chinese PLA General Hospital, ³Stem Cell and Regenerative Medicine Lab,Beijing Institute of Radiation Medicine

Journal (Developmental Biology)

Inducing 3D Neural Retina Formation from Cultured H9-Embryonic Stem Cells for Stem-Cell Based Therapies

Wen Li*^1,2, Hongyu Li*³, Hao Yan, Lixiong Gao, Xiaoling Wang, Lingping Zhao, Yuhan Yan², Zi Ye, Jiafei Xi, Wen Yue, Zhaohui Li²

¹Medical School of Chinese PLA, ²Senior Department of Ophthalmology,The Third Medical Center of Chinese PLA General Hospital, ³Stem Cell and Regenerative Medicine Lab,Beijing Institute of Radiation Medicine

Journal (Developmental Biology)

Generating Neural Retina from Human Pluripotent Stem Cells

Wen Li*^1,2, Hongyu Li*³, Hao Yan, Lixiong Gao, Xiaoling Wang, Lingping Zhao, Yuhan Yan², Zi Ye, Jiafei Xi, Wen Yue, Zhaohui Li²

¹Medical School of Chinese PLA, ²Senior Department of Ophthalmology,The Third Medical Center of Chinese PLA General Hospital, ³Stem Cell and Regenerative Medicine Lab,Beijing Institute of Radiation Medicine

Journal (Developmental Biology)

Preparation of Mouse Pluripotent Stem Cells (mPSCs) for Transfection

Kieran Maheden, Karen Hwang, Ipek Egilmez, Nika Shakiba

¹School of Biomedical Engineering,University of British Columbia

Journal (Developmental Biology)

An Optimized Mouse Embryonic Stem Cell Based Reverse Poly-Transfection Technique for Rapid Exploration of Nucleic Acid Ratios

Kieran Maheden, Karen Hwang, Ipek Egilmez, Nika Shakiba

¹School of Biomedical Engineering,University of British Columbia

Journal (Developmental Biology)

Reverse and Forward Transfection of Mouse Pluripotent Stem Cells (mPSCs)

Kieran Maheden, Karen Hwang, Ipek Egilmez, Nika Shakiba

¹School of Biomedical Engineering,University of British Columbia

Journal (Developmental Biology)

Flow Cytometry Analysis of Transfected Mouse Pluripotent Stem Cells (mPSCs)

Kieran Maheden, Karen Hwang, Ipek Egilmez, Nika Shakiba

¹School of Biomedical Engineering,University of British Columbia

Journal (Developmental Biology)

Monitoring the Mechanical Evolution of Tissue During Neural Tube Closure of Chick Embryo

Chenjun Shi, Chenchen Handler, Haden Florn, Jitao Zhang

¹Department of Biomedical Engineering, College of Engineering,Wayne State University, ²Fischell Department of Bioengineering,University of Maryland

Journal (Developmental Biology)

Experimental Preparation and Ex Ovo Culture of Chicken Embryo for Brillouin Microscopy

Chenjun Shi, Chenchen Handler, Haden Florn, Jitao Zhang

¹Department of Biomedical Engineering, College of Engineering,Wayne State University, ²Fischell Department of Bioengineering,University of Maryland

Journal (Developmental Biology)

Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation

Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center - Sophia Children's Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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