SUNY Oswego 4 articles published in JoVE Environment Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy Jessica Gibbons1, Poongodi Geetha-Loganathan1 1Department of Biological Sciences, SUNY Oswego Here, we present detailed processing protocols for imaging delicate tissue samples using scanning electron microscopy (SEM). Three different processing methods, namely, hexamethyl disilazana (HMDS) chemical drying, simple air drying, and critical point drying are described for preparing rigid eggshells, embryos at early developmental stages, and fungal cultures respectively. Chemistry Inkjet Printing All Inorganic Halide Perovskite Inks for Photovoltaic Applications Dylan Richmond1, Mason McCormick2, Thilini K. Ekanayaka3, Jacob D. Teeter2, Benjamin L. Swanson1, Nicole Benker3, Guanhua Hao3, Sharmin Sikich4, Axel Enders5, Alexander Sinitskii2, Carolina C. Ilie1, Peter A. Dowben3, Andrew J. Yost3 1Department of Physics, State University of New York-Oswego, 2Department of Chemistry, University of Nebraska-Lincoln, 3Department of Physics, University of Nebraska-Lincoln, 4Department of Chemistry, Doane University, 5Physikalisches Institut, Universität Bayreuth A protocol for synthesizing inorganic-lead-halide hybrid perovskite quantum dot inks for inkjet printing and the protocol for preparing and printing the quantum dot inks in an inkjet printer with post characterization techniques are presented. Biology Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation Yulia Artemenko1, Peter N. Devreotes2 1Department of Biological Sciences, SUNY Oswego, 2Department of Cell Biology, Johns Hopkins University School of Medicine Here we describe methods for assessing cellular response to acute mechanical stimulation. In the microscopy-based assay, we examine localization of fluorescently-labeled biosensors following brief stimulation with shear flow. We also test activation of various proteins of interest in response to acute mechanical stimulation biochemically. Developmental Biology Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions Melinda L. Koyle1, Madeline Veloz1, Alec M. Judd1, Adam C.-N. Wong2,4, Peter D. Newell2,5, Angela E. Douglas2,3, John M. Chaston1,2 1Department of Plant and Wildlife Sciences, Brigham Young University, 2Department of Entomology, Cornell University, 3Department of Molecular Biology and Genetics, Cornell University, 4Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, 5Biological Sciences, SUNY Oswego A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.