Department of Molecular Medicine 2 articles published in JoVE Cancer Research Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency Amani Alshaikh1,2, Dmytro Grygoryev3, Dove Keith4, Brett Sheppard4,5, Rosalie C Sears4,6, Jungsun Kim3,6, Abdenour Soufi1 1Centre for Regenerative Medicine, Institute of Regeneration and Repair, Institute of Stem Cell Research, The University of Edinburgh, 2King Abdulaziz City for Science and Technology Health Sector (KACST), 3Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health & Science University, 4Brenden-Colson Canter for Pancreatic Care, Oregon Health & Science University, 5Department of Surgery, Oregon Health & Science University, 6Department of Molecular & Medical Genetics, Oregon Health & Science University The present protocol describes the reprogramming of Pancreatic Ductal Adenocarcinoma (PDAC) and normal pancreatic ductal epithelial cells into induced pluripotent stem cells (iPSCs). We provide an optimized and detailed, step-by-step procedure, from preparing lentivirus to establishing stable iPSC lines. Developmental Biology Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants Megan Vaughan1, Katja A. Lamia1 1Scripps Research, Department of Molecular Medicine Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.
