International School for Advanced Studies View Institution's Website 2 articles published in JoVE Neuroscience Two-Photon Polymerization 3D-Printing of Micro-scale Neuronal Cell Culture Devices Ali Hosseini1, Giovanni Noselli2, Michele Giugliano1 1Neuroscience Department, International School for Advanced Studies, 2Mathematics Department, International School for Advanced Studies 3D printing at the micrometer scale enables rapid prototyping of polymeric devices for neuronal cell cultures. As a proof-of-principle, structural connections between neurons were constrained by creating barriers and channels influencing neurite outgrowth, while the functional consequences of such manipulation were observed by extracellular electrophysiology. Neuroscience Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons Anna Boccaccio1,2, Claudia Sagheddu1, Anna Menini1,3 1SISSA, International School for Advanced Studies, 2Istituto di Biofisica, Consiglio Nazionale delle Ricerche, 3SISSA Unit, Italian Institute of Technology Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.
