KBRwyle GmbH 1 article published in JoVE Neuroscience Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice Andreas Lundt1,2, Julien Soos1, Christina Henseler1, Muhammad Imran Arshaad1, Ralf Müller3, Dan Ehninger4, Jürgen Hescheler5, Agapios Sachinidis5, Karl Broich6, Carola Wormuth1,7, Anna Papazoglou1, Marco Weiergräber1 1Experimental Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, 2KBRwyle GmbH, 3Cognitive Neurophysiology, Department of Psychiatry and Psychotherapy, Faculty of Medicine, University of Cologne, 4Molecular and Cellular Cognition, German Center for Neurodegenerative Diseases (DZNE), 5Institute of Neurophysiology, Faculty of Medicine, University of Cologne, 6Federal Institute for Drugs and Medical Devices (BfArM), 7Thescon GmbH Brainstem evoked response audiometry is an important tool in clinical neurophysiology. Nowadays, brainstem evoked response audiometry is also applied in the basic science and preclinical studies involving both pharmacological and genetic animal models. Here we provide a detailed description of how auditory brainstem responses can be successfully recorded and analyzed in mice.