Center for Behavioral Brain Sciences (CBBS) 1 article published in JoVE Behavior Combined Shuttle-Box Training with Electrophysiological Cortex Recording and Stimulation as a Tool to Study Perception and Learning Max F.K. Happel1,2, Matthias Deliano*1, Frank W. Ohl*1,2,3 1Leibniz Institute for Neurobiology, Magdeburg, Germany, 2Otto-von-Guericke University, Magdeburg, Germany, 3Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany Shuttle-box avoidance learning is well-established in behavioral neuroscience. This protocol describes how shuttle-box learning in rodents can be combined with site-specific electrical intracortical microstimulation (ICMS) and simultaneous chronical in vivo recordings as a tool to study multiple aspects of learning and perception.