Stomatological Hospital of Chongqing Medical University 1 article published in JoVE Immunology and Infection Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis Yan Zhang*1, Xingxing Su*2, Lisha Wang2, Zhengliang Yue2, Qiao Liu3, Ling Ran2, Shun Lei2, Jianjun Hu2, Lifan Xu2, Lilin Ye2, Ping Ji4, Guimei Li1, Qizhao Huang5, Shuqiong Wen4 1College of Veterinary Medicine, Qingdao Agricultural University, 2Institute of Immunology, Third Military Medical University, 3Institute of Cancer, Xinqiao Hospital, Third Military Medical University, 4Stomatological Hospital of Chongqing Medical University, 5Institute of life science, Chongqing Medical University The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8+ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8+ T cells.