Journal
Biochemie
A Simple Assay to Measure ER-Mitochondria Contacts for High-Throughput Screens

A Simple Assay to Measure ER-Mitochondria Contacts for High-Throughput Screens

October 11, 2024

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts - Implications for Neurodegenerative Disease Pathogenesis

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1Department of Pathology,Cedars-Sinai Medical Center, 2Department of Biomedical Science Education, College of Medicine,Charles R. Drew University of Medicine and Science

Summary

We have established a split-luciferase reassembly assay to monitor the endoplasmic reticulum-mitochondria contacts in live cells. Using this assay, we describe a protocol to quantitatively measure the level of these inter-organelle couplings in HEK293T cells, under the condition of chemical treatment.

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