Microfluidic Droplet Assay for Single-Cell Cytokine Secretion Analysis
1Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences,ETH Zürich, 2Department of Biomedicine,Aarhus University, 3Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology,ETH Zürich
Summary
The protocol describes an advanced microfluidic platform to quantitatively measure cytokine secretion dynamics of individual human peripheral blood mononuclear cells. The platform measures up to three cytokines in parallel (IL-6, TNFα, and IL-1β) for each individual cell stimulated with lipopolysaccharide as an example.
