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Optimizing Stereotaxic Microinjection Accuracy in Mice

Optimizing Stereotaxic Microinjection Accuracy in Mice

July 19, 2024

通过冷冻切片初步验证立体定向注射坐标

小鼠靶向脑区显微注射溴酚染料

小鼠立体定向手术后的脑组织采集、冷冻切片和成像

Xiaoxin Zhou*^1,2,3, Wanbing Dai*^1,2, Jie Zhou*^1,2, Yizhe Zhang^1,2, Xiao Zhang^1,2, Sihan Chen^1,2, Xiaoyu Sun^1,2

¹Department of Anesthesiology, Renji Hospital,Shanghai Jiaotong University, School of Medicine, ²Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education, ³Department of Anesthesiology, Shanghai General Hospital,Shanghai Jiaotong University

Summary

本方案描述了一种实用策略，用于在使用染料和冷冻切片进行病毒示踪之前加快立体定位注射坐标的验证步骤。

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