1Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China,the Second Affiliated Hospital, Guangzhou Medical University, 2The Second Clinical Medicine School of Guangzhou Medical University, 3Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University, 4Key Laboratory for Reproductive Medicine of Guangdong Province,The Third Affiliated Hospital of Guangzhou Medical University
Summary
Aquí, presentamos un protocolo para la obtención de imágenes de calcio ex vivo en Drosophila adulta que expresa GCaMP6 para monitorear las actividades epileptiformes. El protocolo proporciona una herramienta valiosa para investigar eventos ictales en Drosophila adulta a través de imágenes de calcio ex vivo , lo que permite explorar los posibles mecanismos de la epilepsia a nivel celular.
