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Journal
Biochemie
Isolating Small Extracellular Vesicles and Extracting Their Peptidome
Isolating Small Extracellular Vesicles and Extracting Their Peptidome
June 30, 2023
骨髄由来マクロファージ由来の微小細胞外小胞ペプチドの同定
01:19 min
•
1,018 Views
示差超遠心法による微小細胞外小胞(sEV)の単離
04:25 min
•
406 Views
単離された小細胞外小胞(sEV)からのペプチドの抽出
03:04 min
•
296 Views
Jiale Cheng
1,2
,
Jiong Zhu
1,2
,
Yujiao Liu
2,3
,
Chen Yang
2,3
,
Yuehui Zhang
2
,
Yuchen Liu
2
,
Chaozhi Jin
2
,
Jian Wang
1,2
1
School of Basic Medical Sciences
,
Anhui Medical University
,
2
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing)
,
Beijing Institute of Lifeomics
,
3
School of Life Sciences
,
Hebei University
Summary
このプロトコルは、示差超遠心によってマクロファージから小さな細胞外小胞を単離し、質量分析による同定のためにペプチドームを抽出する手順を説明しています。
Article
