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Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

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Author Spotlight: Optimizing Grid Preparation for Enhanced Cryoelectron Tomography

JoVE 杂志
生物化学

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JoVE 杂志生物化学

Sample Preparation for In Situ Cryotomography of Mammalian Cells

Author Spotlight: Optimizing Grid Preparation for Enhanced Cryoelectron Tomography

Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

DOI:

10.3791/65697-v

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01:19 min

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December 15, 2023

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Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Tags

Sample Preparation In Situ Cryotomography Mammalian Cells Electron Microscopy Grid Preparation Cell Attachment Cellular Protrusions Specialized Equipment Micro Patterning 3D Printed Holders Cellular Biology Virology Correlative Imaging

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