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Generation of 3D Tumor Spheroids for Drug Evaluation Studies
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癌症研究
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JoVE 杂志 癌症研究
Generation of 3D Tumor Spheroids for Drug Evaluation Studies

Generation of 3D Tumor Spheroids for Drug Evaluation Studies

DOI:
10.3791/65125-v

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10:33 min

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February 24, 2023

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, , , , , , ,
1Department of Oncology, School of Medicine,Southeast University, 2State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering,Southeast University, 3Institute of Medical Devices (Suzhou),Southeast University, 4Jiangsu Avatarget Biotechnology Co., Ltd.

Chapters

  • 00:04Introduction
  • 00:58Spheroid Construction
  • 05:38Drug Treatment and Spheroid Viability
  • 07:09Spheroid Observation and Deep Learning Analysis Through Images in the Drug Test
  • 08:23Results: Brightfield Images of NCI‐H23 Cell Spheroids Treated with AMG510, Tumor Spheroid Viability, Tumor Size and Invasiveness
  • 10:09Conclusion

Summary

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This article demonstrates a standardized method for constructing three-dimensional tumor spheroids. A strategy for spheroid observation and image-based deep-learning analysis using an automated imaging system is also described.

Tags

3D Tumor SpheroidsDrug EvaluationHigh-throughput ImagingHigh-content AnalysisAMG510NCI-H23 CellsAnti-adhesion ReagentTrypsin-EDTACell SuspensionCell Seeding DensityCentrifugation

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