Journal
/
生物化学
/
Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

DOI:
10.3791/64775-v

•

07:14 min

•

June 16, 2023

•

, , , , , , , ,
1Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine

Chapters

  • 00:04Introduction
  • 01:03Collection, Preparation, and Extraction of Plasma Sample
  • 03:28ELISA Plate Preparation (Blocking)
  • 04:16Enzyme-Linked Immunosorbent Assay (ELISA)
  • 06:06Results: Effect of Blocking on CGRP Quantification
  • 06:29Conclusion

Summary

自动翻译

自动翻译

Published data pertaining to calcitonin gene-related peptide (CGRP) concentrations in human plasma are inconsistent. These inconsistencies may be due to the lack of a standardized, validated methodology to quantify this neuropeptide. Here, we describe a validated enzyme-linked immunosorbent assay (ELISA) protocol to purify and quantify CGRP in human plasma.

Tags

Keywords: CGRPEnzyme-linked Immunosorbent AssayELISAPlasmaPurificationExtractionQuantificationMigraineBiomarker

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/64775?article_type=v

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
check_url/cn/64775?article_type=v

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
check_url/cn/64775?article_type=v

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
check_url/cn/64775?article_type=v

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
check_url/cn/64775?article_type=v

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
check_url/cn/64775?article_type=v

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
check_url/cn/64775?article_type=v

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
check_url/cn/64775?article_type=v

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
check_url/cn/64775?article_type=v

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
check_url/cn/64775?article_type=v

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Read Article