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小鼠胸腺细胞在δ样4表达基质细胞上的共培养和转导以研究T细胞白血病的癌基因

JoVE 杂志
癌症研究

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JoVE 杂志癌症研究

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

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小鼠胸腺细胞在δ样4表达基质细胞上的共培养和转导以研究T细胞白血病的癌基因

DOI:

10.3791/64271-v

•

09:43 min

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June 09, 2023

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Gisele O. L. Rodrigues, WenQing Li, Sarah D. Cramer^2,3, Hila Y. Winer, Tu Chun Hsu^2,3, Timothy Gower, Julie A. Hixon, Scott K. Durum

¹Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute,National Institutes of Health, ²Comparative Biomedical Scientist Training Program,NIH, ³Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory,Michigan State University, ⁴NCI-Frederick Laboratory Animal Sciences Program

Chapters

00:04Introduction
00:47Seeding and Transfecting the Retrovirus-Producer Cells
02:31Thymocyte Preparation and Depletion of CD4⁺ and CD8⁺ Cells
04:53Harvesting Retrovirus and Transducing the Thymocytes
07:13Results: Thymocyte Depletion and Thymocyte Retroviral Transduction Efficiency
08:51Conclusion

Summary

自动翻译

该协议描述了从小鼠胸腺中分离双阴性胸腺细胞，然后在δ样4表达骨髓基质细胞系共培养系统（OP9-DL4）上进行逆转录病毒转导和共培养以进行进一步的功能分析。

Tags

Co-culture Transduction Murine Thymocytes Delta-Like 4 Stromal Cells T-cell Leukemia Retroviral Vectors OP9 DL4 In Vitro System T-cell Development Cancer Thymocyte Suspension Thymus PBS Glass Slides Cell Filtration

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