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传染性单核细胞增多症儿童外周血样本中免疫细胞亚群的分离
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08:44 min
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September 07, 2022
DOI:
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016,Chinese Academy of Medical Sciences
Summary
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我们描述了一种结合免疫磁珠和荧光激活细胞分选的方法，以分离和分析外周血单核细胞（单核细胞，CD4 + T细胞，CD8 + T细胞，B细胞和自然杀伤细胞）的已定义免疫细胞亚群。使用这种方法，可以纯化和分析磁性和荧光标记的细胞。
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Cite this Article
Zhang, L., Liu, M., Zhang, M., Ai, J., Tian, J., Wang, R., Xie, Z. Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis. J. Vis. Exp. (187), e64212, doi:10.3791/64212 (2022).
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