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1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine,Jinan University, 2Department of Clinical Pathology,First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine,Florida State University
Chapters
- 00:05Introduction
- 00:50Egg Preparation
- 02:30Egg Windowing and Embryo Identification
- 03:39Neural Tube Injection and Electroporation
- 06:20Otocyst Injection and Electroporation
- 07:44Results: FMRP Knockdown in the Chicken NM and Auditory Duct and Axonal Tracking of Transfected AG Neurons in the Brainstem
- 10:18Conclusion
Usando electroporación in ovo , ideamos un método para transfectar selectivamente el oído interno auditivo y el núcleo coclear en embriones de pollo para lograr una eliminación específica del grupo celular de la proteína de retraso mental X frágil durante períodos discretos de ensamblaje del circuito.
