Journal
/
神经科学
/
Dissekere celle-autonom funksjon av skjøre X mental retardasjon protein i en auditiv krets ved i ovo elektroporering
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

Dissekere celle-autonom funksjon av skjøre X mental retardasjon protein i en auditiv krets ved i ovo elektroporering

DOI:
10.3791/64187-v

•

11:10 min

•

July 06, 2022

•

, , ,
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine,Jinan University, 2Department of Clinical Pathology,First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine,Florida State University

Chapters

  • 00:05Introduction
  • 00:50Egg Preparation
  • 02:30Egg Windowing and Embryo Identification
  • 03:39Neural Tube Injection and Electroporation
  • 06:20Otocyst Injection and Electroporation
  • 07:44Results: FMRP Knockdown in the Chicken NM and Auditory Duct and Axonal Tracking of Transfected AG Neurons in the Brainstem
  • 10:18Conclusion

Summary

自动翻译

自动翻译

Ved hjelp av ovo-elektroporering utviklet vi en metode for selektivt å transfektere det hørbare indre øret og cochleakjernen i kyllingembryoer for å oppnå en cellegruppespesifikk knockdown av skjørt X-mentalt retardasjonsprotein i diskrete perioder med kretsmontering.

Tags

Keywords: In Ovo ElectroporationCell-autonomous FunctionFMRPFragile X SyndromeAuditory CircuitStage-specificSite-specificDirection-specificFmr1 KnockdownDrug-inducible VectorEmbryo ManipulationHH StageAlbumen RemovalWindowingInk Injection

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/64187?article_type=v

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
check_url/cn/64187?article_type=v

TMS: Using the Theta-Burst Protocol to Explore Mechanism of Plasticity in Individuals with Fragile X Syndrome and Autism
check_url/cn/64187?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/cn/64187?article_type=v

I ovo electroporation i Chick midbrain for å studere gen-funksjon i dopaminerg Neuron Development
check_url/cn/64187?article_type=v

I ovo Electroporation av miRNA-baserte Plasmids i Utvikling Neural Tube og vurdering av fenotyper av Dii Injeksjon i åpen bok Forberedelser
check_url/cn/64187?article_type=v

Undersøkelser på Endringer i Hippocampus Circuit Funksjon Etter mild traumatisk hjerneskade
check_url/cn/64187?article_type=v

En lett, hodetelefoner-basert system for å manipulere Auditiv tilbakemelding i Songbirds
check_url/cn/64187?article_type=v

Påvisning av Protein palmitoylation i Cultured hippocampus neurons ved Immunoutfellingsanalyse og Acyl-Biotin Exchange (ABE)
check_url/cn/64187?article_type=v

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
check_url/cn/64187?article_type=v

Brain Slice Biotinvlerina: An<em> Ex Vivo</em> Approach to Measure Region spesifikke Plasma Membrane Protein Trafficking in Adult Neuroner

Read Article