需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Chapters
- 00:04Introduction
- 01:13Loading the Sample
- 02:06Injecting the Oocytes
- 02:49Recording of Ion Currents Using a Two-Electrode Voltage Clamp
- 06:50Analyzing TEVC Recordings
- 07:30Results: Representative Ion Currents of Oocytes Microtransplanted with Human Synaptic Receptors and Polarized Insertion of Receptors
- 09:25Conclusion
Le protocole démontre qu’en effectuant une microtransplantation de membranes synaptiques en ovocytes Xenopus laevis , il est possible d’enregistrer des réponses cohérentes et fiables de l’acide α-amino-3-hydroxy-5-méthyl-4-isoxazolepropionique et des récepteurs de l’acide γ-aminobutyrique.
Tags
MicrotransplantationSynaptic MembranesSynaptic ReceptorsFunctional StudiesNeurodegenerative DisordersMental Health DisordersSynaptic CommunicationSynaptic ReceptorsBrain ReceptorsReceptor FunctionalityReceptor ChangesReceptor ActivityInjectionNanoinjectorOocytesSample PreparationSonicationThermoplasticManipulatorFoot PedalPetri DishControl Group
