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原位 冷冻切片斑马鱼胚胎中的杂交结合免疫组织化学
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07:36 min
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March 03, 2022
DOI:
1School of Life Sciences,Nantong University, 2Medical School,Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration,Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center,Nantong University
Summary
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该协议描述了如何通过结合斑马鱼胚胎切片的 原位 杂交和免疫组织化学来获取图像。在冷冻切片之前进行 原位 杂交，然后进行抗体染色。如果抗体缺乏，检测斑马鱼中两个基因的表达模式是有用的。
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Cite this Article
Wang, J., Chai, R., Fang, X., Gu, J., Xu, W., Chen, Q., Chen, G., Zhu, S., Jin, Y. In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos. J. Vis. Exp. (181), e63715, doi:10.3791/63715 (2022).
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