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Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
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生物化学
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JoVE 杂志 生物化学
Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays

Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays

DOI:
10.3791/63630-v

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12:12 min

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March 29, 2022

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, , ,
1Instituto de Biotecnología,Universidad Nacional Autónoma de México, 2Departamento de Micro y Nanotecnologías,Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México

Chapters

  • 00:04Introduction
  • 01:29Organism Collection and Tissue Hydration
  • 02:26Toxin Release
  • 03:21Quantitation of Total Protein
  • 04:03Determine Polypeptide Venom Complexity and Protein Staining
  • 05:24Prepare Red Blood Cell Solution
  • 06:31Hemolysis Assay
  • 07:24Phospholipase Assay
  • 08:46Results: Nematocyst Discharge, Electrophoretic Profile of Sea Anemone Crude Extract, Hemolysis Assay in Erythrocytes from Sheep, and Phospholipase Assay
  • 11:01Conclusion

Summary

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Here, we describe a protocol to obtain crude venom extract from sea anemone and detect its hemolytic and phospholipase activity.

Tags

Keyword Extraction: Hemolytic ActivityPhospholipase ActivitySea Anemone ToxinBioassaysCrude ExtractProteinHemolysisPhospholysisErythrocyteTemperatureLyophilizationCell LysisNematocyst DischargeCentrifugationBradford Colorimetric Assay

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