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1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation,Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences,Center for Drug Evaluation and Research, Food and Drug Administration
Chapters
- 00:04Introduction
- 01:05Permeable Support Seeding
- 05:30TEER Value Measurement
- 06:51Experimental Layout
- 08:09Results: Staining, Light Microscopy, and TEM Images of Canine Intestinal Organoid Monolayer
- 10:36Conclusion
Qui, presentiamo un protocollo che descrive la coltura di organoidi intestinali canini in un sistema di supporto permeabile a doppia camera. Vengono descritti la semina organoide nei supporti permeabili, il mantenimento del monostrato e i successivi esperimenti di permeabilità ai farmaci.
Tags
Canine Intestinal OrganoidsDual-chamber Permeable Support SystemBiomedical Research ModelsOral PermeabilityTherapeutic Drug CandidatesIn Vitro Tool2D Cell Lines3D Self-assembled Epithelial StructuresAdult Stem CellsMonolayer MaintenanceExtracellular MatrixTight JunctionsCell DifferentiationNuclear ReceptorsCaco-2MDCKGastrointestinal AnatomyIntestinal MicrofloraDrug PermeabilityParallel Drug Development
