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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
Chapters
- 00:04Introduction
- 01:27Grid Loading, Mapping, and Selection of Best Cryo-EM Grids for Data Collection
- 02:36Setting up a Data Collection Session in the Single Particle Analysis Software
- 05:44Final Microscope Alignments Before Starting Data Collection
- 07:15Results: Gallery of Grids, Cryo-EM Reconstruction of Apo-Ferritin, and Effect of Energy Filtering on Cryo-EM Images
- 08:56Conclusion
High-resolution cryo-EM maps of macromolecules can be also achieved by using 200 kV TEM microscopes. This protocol shows the best practices for setting accurate optics alignments, data acquisition schemes, and selection of imaging areas that are all essential for the successful collection of high-resolution datasets using a 200 kV TEM.
