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Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

DOI:
10.3791/63483-v

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09:36 min

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June 23, 2022

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, , ,
1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”,University of Belgrade Faculty of Biology

Chapters

  • 00:04Introduction
  • 01:04Primary Cell Culture Preparation
  • 04:21Microglial Cell Culture
  • 05:26Time-Lapse Video Imaging
  • 06:36Data Analysis
  • 07:34Results: Characterization of Different Glial Cell Types in Culture
  • 08:43Conclusion

Summary

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We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca2+ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1G93A rat model.

Tags

Keywords: Primary Astrocyte CulturePrimary Microglia CultureCalcium SignalingAmyotrophic Lateral SclerosisNeuroinflammatory DiseasesPersonalized MedicineCell IsolationCell CultureDMEMFBSAntibiotics

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