需要订阅 JoVE 才能查看此. 登录或开始免费试用。
A Modified Surgical Technique for Kidney Transplantation in Mice
1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery,Chengdu Women’s and Children’s Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue,The first affiliated Hospital of Hainan Medical University, 4Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases,Hannover Medical School, 5Department of Nephrology,Hannover Medical School, 6Department of Nephrology, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg, 7Department of Dermatology, University Hospital Erlangen,Friedrich-Alexander University Erlangen-Nürnberg
Chapters
- 00:05Introduction
- 01:09Preparatory Steps
- 01:43Donor Operation Procedure
- 03:26Recipient Operation Procedure
- 07:29Ureteral Implantation
- 09:19Representative Results
- 10:12Conclusion
This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.
