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Engineered Lung Tissues Prepared from Decellularized Lung Slices
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Engineered Lung Tissues Prepared from Decellularized Lung Slices

Engineered Lung Tissues Prepared from Decellularized Lung Slices

2,998 Views

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08:01 min

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January 21, 2022

DOI:

10.3791/63151-v

DOI:
10.3791/63151-v

•

08:01 min
January 21, 2022

•

25 Views
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1Department of Biomedical Engineering,Yale University, 2Yale School of Medicine, 3Department of Cellular and Molecular Physiology,Yale School of Medicine, 4Department of Anesthesiology,Yale School of Medicine

Summary

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This protocol describes a method to generate reproducible, small-scale engineered lung tissues, by repopulating decellularized precision-cut lung slices with alveolar epithelial type 2 cells, fibroblasts, and endothelial cells.

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Cite this Article

Leiby, K. L., Ng, R., Campbell, S. G., Niklason, L. E. Engineered Lung Tissues Prepared from Decellularized Lung Slices. J. Vis. Exp. (179), e63151, doi:10.3791/63151 (2022).

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