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微图案透射电子显微镜网格，用于在全细胞冷冻电子断层扫描工作流程中指导细胞定位
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September 13, 2021
DOI:
1Department of Biochemistry,University of Wisconsin, Madison, 2Cryo-Electron Microscopy Research Center, Department of Biochemistry,University of Wisconsin, Madison, 3Midwest Center for Cryo-Electron Tomography, Department of Biochemistry,University of Wisconsin, Madison, 4Department of Chemistry,University of Wisconsin, Madison, 5Morgridge Institute for Research
Summary
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该协议的目标是将细胞粘附和生长引导到冷冻电子显微镜的网格目标区域。这是通过应用抗污垢层来实现的，该抗污垢层以用户指定的模式消融，然后在细胞接种之前在图案化区域沉积细胞外基质蛋白。
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Sibert, B. S., Kim, J. Y., Yang, J. E., Wright, E. R. Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows. J. Vis. Exp. (175), e62992, doi:10.3791/62992 (2021).
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