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Beeldvorming mitochondriale Ca2+ opname in astrocyten en neuronen met behulp van genetisch gecodeerde Ca2+ indicatoren (GECI's)
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)
DOI:

07:46 min

January 22, 2022

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Chapters

  • 00:03Introduction
  • 00:38Construction of DNA Plasmids
  • 00:57In vitro Mitochondrial Ca2+ Imaging in Astrocytes and Neurons
  • 01:32In vivo Mitochondrial Ca2+ Imaging in Astrocytes and Neurons
  • 03:51Cranial Window Installation and in vivo 2-P Imaging of Mitochondrial Ca2+ Signals
  • 05:42Representative Results of in vitro and in vivo Mitochondrial Ca2+ Imaging
  • 07:24Conclusions

Summary

自动翻译

Dit proctocol heeft tot doel een methode te bieden voor in vitro en in vivo mitochondriale Ca2+ beeldvorming in astrocyten en neuronen.

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