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从斑马鱼胚胎中产生幼稚的胚泡外植体
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Generation of Naïve Blastoderm Explants from Zebrafish Embryos
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从斑马鱼胚胎中产生幼稚的胚泡外植体

3,013 Views

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07:21 min

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July 30, 2021

DOI:

10.3791/62797-v

DOI:
10.3791/62797-v

•

07:21 min
July 30, 2021

•

2 Views
,
1Center for Precision Environmental Health and Department of Molecular and Cellular Biology,Baylor College of Medicine

Summary

Automatically generated

斑马鱼胚泡外植体是通过从早期胚胎内的内源性信号中心分离胚胎细胞而产生的，产生相对幼稚的细胞簇，易于操纵和 离体培养。本文提供了制造这种外植体的说明，并通过询问原肠胚形成过程中节点信号的作用来证明它们的效用。

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Cite this Article

Alaniz Emig, A., Williams, M. L. K. Generation of Naïve Blastoderm Explants from Zebrafish Embryos. J. Vis. Exp. (173), e62797, doi:10.3791/62797 (2021).

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