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Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
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工程学
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JoVE 杂志 工程学
Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

DOI:
10.3791/62581-v

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07:34 min

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August 05, 2021

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, , , , , , ,
1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering,Ohio State University, 2Center for Regenerative Medicine,Nationwide Children’s Hospital, 3Thermo Fisher Scientific

Chapters

  • 00:04Introduction
  • 00:50Pulmonary Valve Excision
  • 02:00Pressure Fixation of Pulmonary Valve
  • 03:19En Bloc Sample Staining and Embedment
  • 05:48Results: Correlative Imaging of the Murine Pulmonary Valve
  • 07:10Conclusion

Summary

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Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.

Tags

Murine Pulmonary ValveCorrelative ImagingStructure-functionControlled FixationHigh-resolution ImagingMouse DissectionThoracotomyPulmonary Artery PressurizationPrimary Fixative Solution

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