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Etablering og genmanipulation af Murine Hepatocyte organoider
JoVE 杂志
生物学
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JoVE 杂志 生物学
Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
DOI:

14:54 min

February 12, 2022

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Chapters

  • 00:01Introduction
  • 00:47Murine Hepatocyte Isolation by Liver Perfusion and Digestion
  • 02:47Hepatocyte Organoids Culture
  • 04:29Single Cells from Hepatocyte Organoids
  • 07:22siRNA or Plasmid Transfection
  • 12:58Representative Results
  • 13:58Conclusion

Summary

自动翻译

Et langsigtet ex vitro 3D organoid kultursystem blev etableret fra musehæpatocytter. Disse organoider kan passage og genetisk manipuleres ved lentivirus infektion af shRNA / ektopisk konstruktion, siRNA transfection og CRISPR-Cas9 engineering.

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