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Single Particle Cryo-Electron Microscopy: From Sample to Structure
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

7,406 Views

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11:52 min

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May 29, 2021

DOI:

10.3791/62415-v

DOI:
10.3791/62415-v

•

11:52 min
May 29, 2021

•

20 Views
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1Astbury Centre Structural Molecular Biology, School Molecular and Cellular Biology, Faulty Biological Sciences,University of Leeds, 2Diamond Light Source, Harwell Science and Innovation Campus

Summary

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Structure determination of macromolecular complexes using cryoEM has become routine for certain classes of proteins and complexes. Here, this pipeline is summarized (sample preparation, screening, data acquisition and processing) and readers are directed towards further detailed resources and variables that may be altered in the case of more challenging specimens.

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Cite this Article

White, J. B., Maskell, D. P., Howe, A., Harrow, M., Clare, D. K., Siebert, C. A., Hesketh, E. L., Thompson, R. F. Single Particle Cryo-Electron Microscopy: From Sample to Structure. J. Vis. Exp. (171), e62415, doi:10.3791/62415 (2021).

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