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Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
JoVE 杂志
化学
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JoVE 杂志 化学
Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

DOI:
10.3791/62414-v

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08:35 min

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May 29, 2021

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, , , , , , , , , , , , , ,
1Diamond Light Source Ltd, Harwell Science and Innovation Campus, 2Research Complex at Harwell, Harwell Science and Innovation Campus, 3Structural Genomics Consortium,University of Oxford, 4Centre for Medicines Discovery,University of Oxford, 5Oxford Protein Informatics Group, Department of Statistics,Oxford University, 6Department of Biochemistry,University of Johannesburg

Chapters

  • 00:04Introduction
  • 00:49Fragment Screening Experiment
  • 01:31Crystal Soaking
  • 02:27Crystal Harvesting
  • 03:31Data Collection
  • 04:02Data Analysis
  • 06:47Results: XChem Screen of the Main Protease of SARS‐CoV‐2
  • 07:59Conclusion

Summary

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This paper describes the complete XChem process for crystal-based fragment screening, starting from applying for access and all subsequent steps to data dissemination.

Tags

Keyword Extraction:XChem FacilityDiamond Light SourceCrystal Graphic Fragment ScreeningX-ray CrystallographyFBDDLead DiscoveryXChem PlatformSoaking MethodsData CollectionTextRankEcho SoftwareAcoustic DispenserCrystal Harvesting DeviceLiquid Nitrogen

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