Journal
/
神经科学
/
In vitro kvantitativ billeddannelse assay for phagocytose af døde neuroblastom celler ved iPSC-makrofager
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

In vitro kvantitativ billeddannelse assay for phagocytose af døde neuroblastom celler ved iPSC-makrofager

DOI:
10.3791/62217-v

•

09:48 min

•

February 14, 2021

•

, , , ,
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, 3UK Dementia Research Institute,Cardiff University

Chapters

  • 00:04Introduction
  • 00:41Preparation of Dead SH-SY5Ys
  • 02:03Labeling of Dead SH-SY5Ys with pH-Sensitive Red Fluorescent Dye
  • 03:18Staining of iPSC-Macrophages
  • 04:50Fixed-Cell High-Content Imaging
  • 07:28Results: Imaging, Optimization and Validation of Phagocytosis Assay
  • 09:16Conclusion

Summary

自动翻译

自动翻译

Neurodegenerative sygdomme er forbundet med dysregulerede mikrogliafunktioner. Denne artikel skitserer en in vitro assay af fagocytose af neuroblastom celler ved iPSC-makrofager. Kvantitative udlæsninger af mikroskopi er beskrevet for både live-celle time-lapse imaging og fast celle højindhold billeddannelse.

Tags

Keywords: In VitroQuantitative Imaging AssayPhagocytosisDead Neuroblastoma CellsIPSC-macrophagesNeurodegenerative DiseaseSH-SY5Y CellsCell DissociationFixationPH-sensitive DyeFluorescent DyeMacrophage MediaHoechst 33342

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/62217?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/cn/62217?article_type=v

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
check_url/cn/62217?article_type=v

En kvantitativ Cell Migration Assay for Murine Enteriske Neural Progenitorer
check_url/cn/62217?article_type=v

TIRFM og pH-følsomme GFP-prober til Evaluer Neurotransmitter Vesikel Dynamics i SH-SY5Y neuroblastomaceller: Cell Imaging og Data Analysis
check_url/cn/62217?article_type=v

In vivo genoverførsel til Schwann-celler i Rodent iskiasnerven ved elektroporering
check_url/cn/62217?article_type=v

In Vitro Fagocytose af Myelin resterne af knoglemarv-afledte makrofager
check_url/cn/62217?article_type=v

Assay udvikling for høje indhold kvantificering af Sod1 Mutant Protein samlede dannelse i levende celler
check_url/cn/62217?article_type=v

En roman In Vitro Live-imaging analyse af Astrocyte-medieret fagocytose bruger pH indikator-konjugeret Synaptosomes
check_url/cn/62217?article_type=v

In Vitro Analysen til at studere en sammenlægning af Tau Protein og stof Screening
check_url/cn/62217?article_type=v

Kvantitativ måling af Intratalalt syntetiseret protein i mus

Read Article