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从多萨尔海马的急性切片准备全细胞记录和神经元重建在成人小鼠的登特陀螺

JoVE 杂志
神经科学
Author Produced

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JoVE 杂志神经科学

Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

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从多萨尔海马的急性切片准备全细胞记录和神经元重建在成人小鼠的登特陀螺

DOI:

10.3791/61980-v

•

10:45 min

•

April 03, 2021

•

Gilda Baccini, Sören Brandt, Peer Wulff

¹Institute of Physiology,Christian-Albrechts-University Kiel

Chapters

00:00Introduction
01:02Preparing the Bench for Slicing
03:57Preparation of Dorsal Hippocampal Slices
07:47Results: The Transversal Slice Shows Enhanced Viability Compared to the Coronal Slice
10:10Conclusion

Summary

自动翻译

我们提出了一个协议，准备从小鼠的单体中间海马的急性切片。我们将这种横向制剂与日冕切片在录音质量和记录神经元形态特征的保存方面进行比较。

Tags

Keywords: Acute Slices Dorsal Hippocampus Whole-cell Recording Neuronal Reconstruction Dentate Gyrus Adult Mice Brain Slicing Electrophysiology Video Recordings Transversal Slices Protecting Solution Vibratome Cutting Solution Transcardial Perfusion Incubation Chamber Slice Storage Chamber

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