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研究TGF-β信号和TGF-β引起的乳腺癌和正常细胞的上皮到中位过渡
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癌症研究
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JoVE 杂志 癌症研究
Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
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研究TGF-β信号和TGF-β引起的乳腺癌和正常细胞的上皮到中位过渡

11,496 Views

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06:54 min

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October 27, 2020

DOI:

10.3791/61830-v

DOI:
10.3791/61830-v

•

06:54 min
October 27, 2020

•

15 Views
, , , ,
1Oncode Institute and Department of Cell Chemical Biology,Leiden University Medical Center

Summary

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我们描述了一个系统的工作流程，通过研究这个信号通路中涉及的蛋白质和基因表达来研究TGF-β信号和TGF-β诱导的EMT。这些方法包括西方污渍、露西费拉塞记者检测、qPCR和免疫荧光染色。

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Cite this Article

Zhang, J., Thorikay, M., van der Zon, G., van Dinther, M., ten Dijke, P. Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells. J. Vis. Exp. (164), e61830, doi:10.3791/61830 (2020).

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