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生物化学
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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
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生物化学
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JoVE 杂志 生物化学
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

DOI:
10.3791/61790-v

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06:47 min

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January 28, 2021

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, , , ,
1Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 2School of Biotechnology and Health Sciences,Wuyi University

Chapters

  • 00:05Introduction
  • 00:58Isolation of Plasma Lipoproteins
  • 03:00Results: Analysis of Lipoproteins and their Compositional Apolipoproteins Isolated from Rabbits on a Normal and High Cholesterol Diet
  • 05:32Conclusion

Summary

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Several methods have been used for analyzing plasma lipoproteins; however, ultracentrifugation is still one of the most popular and reliable methods. Here, we describe a method regarding how to isolate lipoproteins from plasma using sequential density ultracentrifugation and how to analyze the apolipoproteins for both diagnostic and research purposes.

Tags

Plasma LipoproteinsUltracentrifugationLipoprotein FractionationHyperlipidemiaAtherosclerosisLipoprotein ProfileHigh Cholesterol DietHDLLDLVLDL

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