Journal
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环境科学
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Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil
JoVE 杂志
环境科学
This content is Free Access.
JoVE 杂志 环境科学
Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil

Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil

DOI:
10.3791/61661-v

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12:03 min

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September 01, 2020

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, , ,
1Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry,Montanuniversität Leoben, 2Department of Forest and Soil Sciences, Institute of Soil Research, Rhizosphere Ecology and Biogeochemistry Group,University of Natural Resources and Life Sciences, Vienna, 3Department of Crop Sciences, Institute of Agronomy,University of Natural Resources and Life Sciences, Vienna

Chapters

  • 00:04Introduction
  • 00:51Diffusive Gradients in Thin Films (DGT) Gel Fabrication and Rhizotron Preparation
  • 03:06Plant Cultivation
  • 03:47Solute Distribution Sampling
  • 04:48Gel Retrieval and Chemical Analysis Preparation
  • 05:38Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) Line Scan Analysis
  • 07:39Data Processing and Calibration
  • 09:03Image Generation
  • 09:48Results: Representative Sub-mm 2D Solute Flux Distribution of Multiple Nutrients and Contaminants Across Different Soil-Root Interfaces
  • 11:24Conclusion

Summary

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This protocol presents a workflow for sub-mm 2D visualization of multiple labile inorganic nutrient and contaminant solute species using diffusive gradients in thin films (DGT) combined with mass spectrometry imaging. Solute sampling and high-resolution chemical analysis are described in detail for quantitative mapping of solutes in the rhizosphere of terrestrial plants.

Tags

Keywords: Two-dimensional VisualizationLabile Inorganic Plant NutrientsContaminantsSoilDGT Mass Spectrometry ImagingNutrient And Contaminant UptakeRhizotronPolyurethane GelPTFE Foil

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