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Produção automatizada de neurônios cortical e dopaminérgico derivados de células-tronco pluripotentes induzidos por humanos com monitoramento integrado de células vivas
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09:34 min
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August 06, 2020
DOI:
1Genome Biology of Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), 2Applied Genomics for Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), 3Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research,University of Tübingen
Summary
Automatically generated
Mostramos a automação das culturas de células-tronco pluripotentes induzidas pelo homem (hiPSC) e diferenciações neuronais compatíveis com imagens e análises automatizadas.
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Automated ProductionHuman Induced Pluripotent Stem CellsCortical NeuronsDopaminergic NeuronsLive-cell MonitoringStandardized ProceduresLow Experimental VariationHigh Phenotypic ReproducibilityGraphic User InterfaceResource Instrument ProcessHEPA HoodDecontaminationUV RadiationLiquid Handling StationCulture MediumDissociation ReagentCell LineProjectBatchSeedingBrightfield Imaging CytometerCell Counting
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Cite this Article
Dhingra, A., Täger, J., Bressan, E., Rodriguez-Nieto, S., Bedi, M., Bröer, S., Sadikoglou, E., Fernandes, N., Castillo-Lizardo, M., Rizzu, P., Heutink, P. Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring. J. Vis. Exp. (162), e61525, doi:10.3791/61525 (2020).
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