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使用SAX-HPLC从不同植物物种中提取和定量可溶性、放射性标记肌醇多磷酸盐
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
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使用SAX-HPLC从不同植物物种中提取和定量可溶性、放射性标记肌醇多磷酸盐

6,441 Views

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09:01 min

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June 26, 2020

DOI:

10.3791/61495-v

DOI:
10.3791/61495-v

•

09:01 min
June 26, 2020

•

18 Views
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1Department of Plant Nutrition, Institute of Crop Science and Resource Conservation,University of Bonn

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Inositol PolyphosphatesRadiolabelingSAX-HPLCPlant MetabolismPlant DevelopmentExtractionQuantificationLotus SeedsMyo-inositolSample PreparationHPLC Setup

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Gaugler, P., Gaugler, V., Kamleitner, M., Schaaf, G. Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC. J. Vis. Exp. (160), e61495, doi:10.3791/61495 (2020).

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