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An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract
Chapters
- 00:04Introduction
- 00:35Mouse Dissection
- 02:33Ex Vivo Prep and Exogenous Addition of Metabolites to the Gut Homogenate Extracts for Hyphal Morphogenesis Assay
- 04:21C. albicans Morphogenesis Assay
- 05:50Results: Effects of Antibiotic and Gut Metabolites on C. albicans Hyphal Morphogenesis
- 06:58Conclusion
The ex vivo assay described in this study using gut homogenate extracts and immunofluorescence staining represents a novel method to examine the hyphal morphogenesis of Candida albicans in the GI tract. This method can be utilized to investigate the environmental signals regulating morphogenetic transition in the gut.
