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09:08 min
October 14, 2021
DOI:
10.3791/61470-v
我们提出了一种简单的方案,用于从诊断为Sézary综合征的患者获得的全血中分离外周血单核细胞,然后选择CD4 + T细胞,用phorbol12-肉豆蔻酸酯13-乙酸盐和A23187离子载体刺激它们,并制备RNA进行转录组学分析。
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Mehdi, S. J., Moerman-Herzog, A. M., Wong, H. K. Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling. J. Vis. Exp. (176), e61470, doi:10.3791/61470 (2021).
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